Or simply because chlorophyll a is synthesized a lot quicker than it’s degraded. Spearman’s correlation coefficients among the expression levels of DEGs and concentrations of chlorophylls aand b ended up calculated for every stage. The expression levels of two and three unigenes were appreciably correlated with chlorophyll a and b concentrations, respectively (Table five). The PORA (unigene 2533) expression levels had been appreciably correlated with chlorophyll a concentrations. The expression of two unigenes (unigene 26042 and unigene 37738) encoding NYC1 was appreciably correlated together with the focus of chlorophylls a and b. These unigenes may be responsible for the dissimilarities while in the biosynthesis of chlorophylls a and bLi et al. BMC Plant Biology (2016) sixteen:Web site eleven ofTable 5 Correlation coefficients among expression levels of differentially expressed chlorophyll biosynthetic pathway unigenes and concentrations of chlorophylls a PRIMA-1 and bUnigene unigene39518 unigene36734 unigene24328 unigene89159 unigene91770 unigene5974 unigene71483 unigene39110 unigene2151 unigene2371 unigene90629 unigene24743 unigene55992 unigene1808 unigene26832 unigene12598 unigene2533 unigene38214 unigene1225 unigene62053 unigene7972 unigene57504 unigene4795 unigene16722 unigene37586 unigene131928 Fig. 6 Theanine biosynthesis pathway plus the connected unigenes. a The glutamine, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/12711626 glutamate, alanine, ethylamine, and theanine concentrations have been identified at distinct levels. The double asterisks reveal a significant distinction between the YG or W phases as well as G stage (P < 0.01; Student's t-test). b Theanine biosynthesis pathway. The bracketed numbers in red following each gene name indicate the number of corresponding unigenes identified in our database. c All differentially expressed genes involved in theanine biosynthesis were hierarchically clustered and mapped using the fragments per kilobase of exon per million mapped read values. Colors indicate the normalized signal intensity as defined in the bar unigene11687 unigene90639 unigene56286 unigene168719 unigene26042 unigene37738 unigene5416 unigene89999 Gene Name HEMA HEMA HEMA HEMA HEMA HEMB HEMB HEMC HEMD HEME HEME CHLD CHLD CHLM CRD CRD PORA PORA PORA PORA PORA PORA CHLG CAO CAO CLH CLH CLH CLH HCAR NYC1 NYC1 PPH PAO Chl a 0.102 0.634 0.498 0.031 0.429 0.123 0.282 0.282 0.23 0.618 0.511 0.634 0.24 0.22 0.339 0.402 0.044* 0.201 0.282 0.201 0.796 0.183 0.121 0.327 0.23 0.308 0.074 0.164 0.25 0.587 0.011* 0.007** 0.012 0.123 Chl b 0.364 0.282 0.25 0.188 0.763 0.123 0.24 0.513 0.158 0.813 0.024 0.897 0.166 0.108 0.364 0.282 0.339 0.603 0.513 0.603 0.376 0.166 0.282 0.22 0.364 0.204 0.351 0.482 0.813 0.415 0.015* 0.01** 0.249 0.*Significant difference at P 0.05; **Significant difference at P 0.among the different leaf color and developmental stages of `Anji Baicha' tea plants.Theanine biosynthesisTheanine synthetase (TS) catalyzes the synthesis of theanine from glutamic acid and ethylamine [57, 58] (Fig. 6b). Additional enzymes involved in theanine biosynthesis include glutamate synthase (GOGAT) and alanine decarboxylase(AIDA), which catalyze the conversion of glutamine to glutamate and alanine to ethylamine, respectively. Glutamine synthetase (GS) is PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9547713 really homologous to TS, and will also catalyze the conversion of glutamic acid and ethylamine to theanine [59]. Ultra-performance liquid chromatography?triple quadrupole mass spectrometry (UPLC qQ-MS) analyses were performed to find out the concentrations of glutamine, gluta.